Listeria Identification Agar 500gm HiMedia

Listeria Identification Agar Base (PALCAM) is recommended for selective isolation and identification of Listeria species from clinical and non-clinical samples. It is also recommended for the isolation of L. monocytogenes from foods. The addition of egg yolk (2.5% v/v) to medium has been reported to aid in the repair of damaged cells. Medium-containing blood when overlaid on the medium enables to differentiation and enumeration of haemolytic Listeria species. On PACAM Agar, colonies of Listeria appear as grey-green with a black precipitate, following inoculation and incubation at 35°C for 24-48 hours under aerobic or microaerophillic conditions.

Listeria Identification Agar Base (PALCAM)

Intended use

Recommended for selective isolation and identification of Listeria species from clinical and non-clinical samples.

Ingredients (Gms / Litre)

• Peptone: 23.000
• Starch: 1.000
• Sodium chloride: 5.000
• Mannitol: 10.000
• Ammonium ferric citrate: 0.500
• Esculin: 0.800
• Dextrose (Glucose): 0.500
• Lithium chloride: 15.000
• Phenol red: 0.080
• Agar: 13.000
• Final pH: ( at 25°C) 7.0±0.2

**Formula adjusted, standardized to suit performance parameters

Directions
Suspend 34.44 grams in 500 ml purified/distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 45-50°C and aseptically add rehydrated contents of 1 vial of Listeria Selective Supplement (PALCAM) (FD061). Mix well and pour into sterile Petri plates. Click for the Technical Data Sheet