Methylene Blue 25 gm – Merck Certistain® is a high-quality laboratory dye widely used in microscopy, bacteriology, and hematology staining techniques. This chemical reagent acts as an overview stain for bacteria and also works as a counterstain in the Ziehl–Neelsen staining method for examining biological samples.
Merck manufactures this product under the Certistain® brand, which represents a range of premium laboratory dyes designed to meet IVD and CE quality standards. As a result, laboratories receive consistent staining performance and reliable diagnostic results.
Additionally, methylene blue plays an important role in hematology because it combines with other dyes such as eosin to prepare various staining solutions used for examining blood cells and microorganisms.
Key Features
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High-Purity Microscopy Dye – Manufactured by Merck under the Certistain® quality range
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Ideal for Bacteriology Staining – Bacteria appear clearly blue under the microscope
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Counterstain for Ziehl–Neelsen Method – Supports acid-fast staining procedures
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Suitable for Hematology Applications – Used in many blood cell staining mixtures
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Reliable Laboratory Performance – Ensures consistent and accurate staining results
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Certified Laboratory Reagent – Meets IVD and CE quality requirements
Chemical Information
| Property | Details |
|---|---|
| Chemical Name | Methylene Blue |
| Synonyms | Methylthioninium chloride, Tetramethylthionine chloride |
| Chemical Formula | C₁₆H₁₈ClN₃S · xH₂O (x = 2–3) |
| Molar Mass | 319.86 g/mol (anhydrous) |
| Color Index | C.I. 52015 |
| HS Code | 3212 90 00 |
| Pack Size | 25 gm |
| Brand | Merck |
| Application | Microscopy staining |
Applications of Methylene Blue 25 gm Merck
Methylene Blue (Certistain®) is widely used in laboratories for:
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Microscopy staining of bacteria
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Ziehl–Neelsen staining method (counterstain)
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Hematology staining procedures
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Microbiological research
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Clinical laboratory analysis
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Educational laboratory experiments
Furthermore, the dye produces a distinct blue coloration, which helps researchers easily identify microorganisms and cellular structures under a microscope.
Safety Information
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Hazard Statement: H302 – Harmful if swallowed
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Handle with appropriate laboratory safety equipment such as gloves and goggles.
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Avoid ingestion or direct contact with skin and eyes.
Storage Instructions
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Store the chemical in a cool and dry place.
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Recommended storage temperature: +2°C to +30°C.
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Keep the container tightly closed and protected from moisture.
✅ Ideal for: microbiology laboratories, pathology labs, hospitals, research institutes, universities, and diagnostic laboratories.


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